NoteApoptosis to HL-60 by Humuione

We found und isolated a strong bonc resorption inhibitor, hurnulone (HU), fi'om hop extract recently.i] 'E'o study the mechanism of the inhibition of bone resorpt{on, we have choscn the preTnyclocytic lcukemia ccli HL-60. Although the target cetl for the humulone molecuge is not clear, one of the candidates for the target cell is osteoclasts. 1'he celE line of osteoclasts has not been established. Osteoclasts derjve from mveloblast stem cells and ditTerentiate finally into mature osteoclasts via the common lineage ofmacrophages. We, therefore. have chosen HL-60 because HL60 is one of' the possible progenitors of osteoclasts, We pianned to study the relationship between bone resorption inhibitor>' activity and apoptosis-inducing actii,ity ot' HU, The structure of HU is shown in Fig. I. HL-60 was grewn in RPMg l64e medium (Gibco BRL) w"h 10U,1 (v,/v) fetal bovine serum (Moregate, Australia}, 2 × 105